alexa fluor 488 conjugated anti mouse igg a11029 (Thermo Fisher)

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Thermo Fisher alexa fluor 488 conjugated anti mouse igg a11029
Alexa Fluor 488 Conjugated Anti Mouse Igg A11029, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a11029/CHOLINE+CHLORIDE+1KG+1KG/pm41734828-59-0-6
Average 97 stars, based on 1 article reviews

alexa fluor 488 conjugated anti mouse igg a11029 - by Bioz Stars, 2026-09

97/100 stars

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Incubation:

Article Title: ENN A1 and B1 In Vitro Toxicological Effects on 2D and 3D Organ-on-Chip HepaRG Liver Cells
Article Snippet: The samples were either incubated with mouse anti-γH2AX antibodies (targeting the phosphorylated histone H2AX at Ser139) (1:250) (05-636, Sigma Aldrich, Louis, MO, USA), mouse anti-Ki67 antibodies (targeting protein Ki-67, expressed during active phases of the cell cycle) (1:600) (94995, CellSignaling, Withby, ON, Canada), or rabbit anti-CC3 antibodies (targeting the activated form of caspase-3) (1:600) (96615, ThermoFisher Scientific, Waltham, MA, USA) overnight at 4 °C. .. After one wash with PBS, the wells or slides were incubated in a secondary antibody buffer (PBS 1X, 3% BSA) with AlexaFluor-647 and AlexaFluor-488 secondary antibodies (both at 1:650) (A21244 and A11029, respectively, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) for 1 h at room temperature. .. The slides or well were stained with DAPI (1:5000), prepared from a stock solution of 5 mg/mL (D3571, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), to label the nuclei.

Article Title: Unveiling a Novel Memory Center in Humans: Neurochemical Identification of the Nucleus Incertus , a Key Pontine Locus Implicated in Stress and Neuropathology
Article Snippet: Sections were washed (3×) in phosphate-buffered saline with 0.1% Tween ® 20 detergent (PBS-T, Cat. No. 20012-027, GIBCO PBS pH 7.2 (1X), Life Technologies Corporation, Grand Island, New York, USA, with Tween ® 20 detergent, Cat. No. P1379, Sigma Aldrich Company, St. Louis, Missouri, USA), and blocked with 3% BSA, with incubation for 1 h. After further washing, sections were incubated in a range of combined primary antisera [RLN3 (1:1,500, Cat. No. PA47448, anti-goat, Invitrogen, Carlsbad, CA, USA) and AT8 (1:500, MN1020, anti-mouse, Invitrogen, Carlsbad, CA, USA), or CRHR1 (1:500, Cat. No. ab150561, anti-rabbit, Abcam, Fremont, CA, USA), or GAD65/67 (1:500, Cat. No. PA5-104543, anti-rabbit, Invitrogen, Carlsbad, CA, USA), or MAP2 (1:500, Cat. No. ab183830, anti-rabbit, Abcam, Fremont, CA, USA), or SST (1:200, Cat. No. ab108456, anti-rabbit, Abcam, Fremont, CA, USA)] antisera; ON at 4°C. .. Sections were washed 3× in PBS-T and incubated in species-specific, fluorophore-conjugated secondary antibodies (1:1,000, Red: Cat. No. A11036, AlexaFluor 568 goat anti-rabbit IgG (H+L) 2 mg/mL, and 1:1,000, Green: Cat. No. A11034, AlexaFluor 488 goat anti-rabbit IgG (H+L) 2 mg/mL, or Cat. No. A11055, donkey anti-goat IgG (H+L) 2 mg/mL, or Cat. No. A11029, goat anti-mouse IgG (H+L) 2 mg/mL, Life Technologies, Eugene, OR, USA). .. After a final wash, sections were mounted, dipped in 1% Sudan Black (Cat. No. 199664, Sigma-Aldrich, St. Louis, Missouri, USA to reduce autofluorescence, and coverslipped with UltraCruz containing DAPI (UltraCruz® Hard-set Mounting Medium, sc-359850, Dallas, TX, USA).

Article Title: Endocytosis and non-canonical autophagy mediate extracellular histones cytotoxicity in vascular models of sepsis
Article Snippet: .. The next day, primary antibody was removed, the cells were washed three times with PBS for 5 minutes and they were incubated with corresponding secondary antibodies (Alexa-Fluor-488-conjugated goat anti-mouse; A11029, Invitrogen, USA) and Alexa-Fluor-Texas Red-conjugated goat anti-rabbit; T862, Invitrogen USA) diluted 1:2000 in blocking solution for 1h at room temperature. .. Finally, the crystals were mounted on slides with DAPI-Fluoromount-G ® mounting medium (Southern Biotech; USA) for nuclei detection and to later be observed under a confocal- or epifluorescence microscope.

other:

Article Title: Detection of ER Stress in iPSC-Derived Neurons Carrying the p.N370S Mutation in the GBA1 Gene
Article Snippet: Secondary antibodies , Goat anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor 488 , 1:400 , Thermo Fisher Scientific Cat. # A11029, RRID:AB_2534088.

Staining:

Article Title: Neutrophil depletion at the early stage of Japanese encephalitis virus infection affects CD8+ T cell infiltration into the mouse brain and causes severe encephalitis
Article Snippet: After blocking, Primary antibody staining for JEV NS3 protein (1:1500) (GTX125868; GeneTex, Irvine, CA, USA) was done for one hour at room temperature (RT). .. Cells were washed multiple times with PBS, Alexa Fluor 488, goat anti-mouse (1:600) (A11029; Invitrogen, Carlsbad, CA, USA) was added for one h at RT, Following multiple wash with 1X PBS, Coverslips were then mounted, and the nucleus was stained with 4′,6-diamidine-2′- pheynylindole dihydrochloride (DAPI) (D1306; Invitrogen, Carlsbad, CA, USA). .. Images were acquired at 40x magnification using a confocal microscope (Leica SP5 Laser Confocal Microscope) and analyzed using ImageJ software.

Saline:

Article Title: Platelets and hemostatic proteins are co-localized with chronic neuroinflammation surrounding implanted intracortical microelectrodes.
Article Snippet: Intracortical microelectrodes induce vascular injury upon insertion into the cortex.. As blood vessels rupture, blood proteins and blood-derived cells (including platelets) are introduced into the ‘immune privileged’ brain tissues at higher-than-normal levels, passing through the damaged blood-brain barrier.. Blood proteins adhere to implant surfaces, increasing the likelihood of cellular recognition leading to activation of immune and inflammatory cells.

Blocking Assay:

FLAG-tag:

Article Title: Small-molecule enhancement of METTL3 S-palmitoylation as a therapeutic strategy for osteoarthritis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-rabbit METTL3 Abcam Cat#ab195352; RRID: AB_2721254 Anti-rabbit Collagen II Abcam Cat#ab307674; RRID: AB_731688 Anti-rabbit Collagen X Abcam Cat#ab260040; RRID: AB_3083555 Anti-rabbit SOX9 Abcam Cat#ab185966; RRID: AB_2728660 Anti-rabbit MMP3 Abcam Cat#ab52915; RRID: AB_881243 Anti-rabbit MMP13 Abcam Cat#ab39012; RRID: AB_776416 Anti-rabbit METTL14 Proteintech Cat# 26158-1-AP; RRID: AB_2800447 Anti-rabbit β-actin Proteintech Cat#81115-1-R; RRID: AB_2923704 Anti-rabbit ACAN Proteintech Cat#13880-1-AP; RRID: AB_2722780 Anti-rabbit Beclin 1 Proteintech Cat#11306-1-AP; RRID: AB_2259061 Anti-rabbit ATG5 Proteintech Cat#10181-2-AP; RRID: AB_2062045 Anti-rabbit P21 Proteintech Cat#110355-1-AP; RRID: AB_2077682 Anti-rabbit P53 Proteintech Cat# 10442-1-AP; RRID: AB_2206609 Anti-rabbit HSC70 Proteintech Cat# 10654-1-AP; RRID: AB_2120153 Anti-rabbit EIF3A Proteintech Cat# 26178-1-AP; RRID: AB_2880413 Anti-rabbit EIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-rabbit RPS6 Proteintech Cat# 80208-1-RR; RRID: AB_2918876 Anti-rabbit FLAG tag Proteintech Cat# 20543-1-AP; RRID: AB_11232216 Anti-rabbit HA-tag Proteintech Cat# 51064-2-AP; RRID: AB_11042321 Anti-rabbit IgG Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-mouse puromycin Sigma-Aldrich Cat# MABE343; RRID: AB_2566826 Anti-rabbit ZDHHC24 Invitrogen Cat# PA5-53461; RRID: AB_2649860 Anti-rabbit Fluorescein Invitrogen Cat# A-889; RRID: AB_221561 Alexa Fluor 488 goat anti-mouse Invitrogen Cat# A11029; RRID: AB_2534088 Alexa Fluor 488 goat anti-rabbit Invitrogen Cat# A11034; RRID: AB_2576217 Alexa Fluor 568 goat anti-mouse Invitrogen Cat# A11031; RRID: AB_144696 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat# A11036; RRID: AB_10563566 Chemicals, peptides, and recombinant proteins D-PBS Stemcell Cat#37350 mTeSR TM 1 Basal Medium Stemcell Cat#354240 mTeSR TM 1 5X Supplement Stemcell Cat#354240 STEMdiff TM Trilineage Differentiation Kit Stemcell Cat# 05230 Gentle Cell Dissociation Reagent Stemcell Cat#100-0485 DMEM/F-12 Stemcell Cat#36254 Dispase Stemcell Cat#07913 Human IL-1 beta Protein MedChemExpress Cat#HY-P73149 Anti-Flag Magnetic Beads MedChemExpress Cat# HY-K0207 Anti-HA Magnetic Beads MedChemExpress Cat# HY-K0201 3-Methyladenine MedChemExpress Cat# 5142-23-4 Cycloheximide MedChemExpress Cat# HY-12320 Copper sulfate anhydrous MedChemExpress Cat# HY-Y1878C Carfilzomib MedChemExpress Cat# HY-10455 (Continued on next page) 18 Cell Reports 45, 116993, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Palmitic acid MedChemExpress Cat# HY-N0830 Chloroquine MedChemExpress Cat#HY-17589A IA-Alkyne MedChemExpress Cat#HY-136205 IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Triton X-100 Sigma-Aldrich Cat# T9284 Polybrene Sigma-Aldrich Cat# H9268 Puromycin Sigma-Aldrich Cat# P9620 Hydroxylamine Sigma-Aldrich Cat# 467804 DTT Sigma-Aldrich Cat# 43815 NH 4 Cl Sigma-Aldrich Cat# A9434 Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# DUO92002 Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H + L) Sigma-Aldrich Cat# DUO92004 Duolink® In Situ Detection Reagents Red Sigma-Aldrich Cat# DUO92008 Duolink® In Situ Wash Buffers, Fluorescence Sigma-Aldrich Cat# DUO82049 Duolink® In Situ Mounting Medium with DAPI Sigma-Aldrich Cat# DUO82040 Tris(2-carboxyethyl) phosphine hydrochloride solution (TCEP) Sigma-Aldrich Cat# 646547 Human TNF-a Protein Sigma-Aldrich Cat# SRP3177 N-Ethylmaleimide Sigma-Aldrich Cat# 04260 Dexamethasone Sigma-Aldrich Cat# D4902 2-Bromohexadecanoic acid Sigma-Aldrich Cat# 21604 Iodoacetamide Sigma-Aldrich Cat# I6125 MMTS Sigma-Aldrich Cat# 208795 DNase I Sigma-Aldrich Cat# 11284932001 Collagenase Type 1 Sigma-Aldrich Cat# LS004197 Insulin-transferrin-selenium (ITS) Invitrogen Cat# 41400045 EDTA Invitrogen Cat# AM9260G NaCl Invitrogen Cat# AM9759 HEPES Invitrogen Cat# 15630130 EDTA decalcifying solution Solarbio Cat# E1171 Toluidine Blue Stain Solution Solarbio Cat# G3660 Ascorbic acid Solarbio Cat#A8100 Corning® Matrigel® hESC-Qualified Matrix Corning Cat# 354277 High Capacity Thiol Reactive Resin Nanocs Cat# AR-SS-2 Alk-16 Click Chemistry Tools Cat# CCT-1165 OG488 Azide Click Chemistry Tools Cat# 1264–1 Biotin BMCC Sangon Biotech Cat# C100222 Nuclease-Free Water Promega Cat# P1199 Critical commercial assays CoraLite®488 Plus TUNELApoptosis Detection Kit I Proteintech Cat# PF00006 Alcian blue Stain Kit Solarbio Cat# G1565 Modified Saffron-O and Fast Green Stain Kit Solarbio Cat# G1371 QuikCyto® Mouse KC/IL-8 ELISA kit Neobioscience Cat# EMC104QT.96 QuikCyto® Mouse IL-6 ELISA kit Neobioscience Cat# EMC004QT.96 QuikCyto® Mouse TNF-α ELISA kit Neobioscience Cat# EMC102aQT.96 m 6 A RNA Methylation Assay Kit Abcam Cat#ab185912 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat# R323-01 (Continued on next page) Cell Reports 45, 116993, March 24, 2026 19

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alexa fluor 488 conjugated anti mouse igg a11029 (Thermo Fisher)

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Incubation:

other:

Staining:

Saline:

Blocking Assay:

FLAG-tag:

Recombinant:

Gentle:

Magnetic Beads:

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